Calcium-activated potassium channels in single smooth muscle cells of rabbit jejunum and guinea-pig mesenteric artery.
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چکیده
منابع مشابه
Single nisoldipine-sensitive calcium channels in smooth muscle cells isolated from rabbit mesenteric artery.
Single smooth muscle cells were enzymatically isolated from the rabbit mesenteric artery. At physiological levels of external Ca, these cells were relaxed and contracted on exposure to norepinephrine, caffeine, or high levels of potassium. The patch-clamp technique was used to measure unitary currents through single channels in the isolated cells. Single channels were selective for divalent cat...
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Effects of intracellularly perfused ATP, and extracellularly applied cyanide and 2-deoxy-D-glucose, on fast and slow Ca2+ channel currents of isolated single vascular smooth muscle cells were investigated by a whole-cell voltage-clamp method combined with an intracellular perfusion technique. Single smooth muscle cells were prepared by collagenase treatment from guinea pig small mesenteric arte...
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Inactivation of currents carried through calcium channels by calcium (ICa), barium (IBa) and monovalent cations (In.s.) was studied in single smooth muscle cell (SMC) of the guinea-pig coronary artery and taenia caeci by the whole-cell patch-clamp method. The rate of ICa inactivation in the coronary artery SMC was correlated with ICa amplitude, and acceleration was observed with the increasing ...
متن کاملSodium conductance in calcium channels of single smooth muscle cells of guinea-pig taenia caeci.
During the last few years, the Ca 2+-channels in isolated smooth muscle cells have been intensively studied (Walsh and Singer 1981; Bury et al. 1982; Klock ner and Isenberg 1985; Droogmans and Callewaert 1986). It was shown that under physiological conditions Ca 2+-channels are not measurably permeable for Na-ions (Klockner and Isenberg 1985). On the other hand, inward currents carried by Na +...
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ATP-sensitive K+ (KATP) channels play an important role in the regulation of smooth muscle membrane potential. To investigate the properties of KATP channels in guinea pig urinary bladder smooth muscle cells, fluorescence-based assays were carried out with the membrane potential-sensitive probe bis-(1,3-dibutylbarbituric acid)trimethine oxonol [DiBAC4(3)]. The prototypical channel openers, incl...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1986
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1986.sp015961